Hybridoma Technologies and Recombinant Antibodies (LRR / HTRP)

Course objectives:
To lern introduced section of hybridoma and recombinant technologies.
Requirements on student
75% correctly answered questions in written test
Content
Immune system, immune response, immunoglobulins, molecular biologie of antibodies, immunochemicals methods, monoclonal, polyclonal and recombinant antibodies, antigen, haptens, methods of conjugation, immunozation, isolation and purification of antibodies; Monoclonal antibodies: hybridoma technologies, characteristic of splenocytes, myeloma cells and hybridomas, growing hybridomas, in vitro and in vivo production methods, immunoassays, biotechnologies, advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies.
Activities
Guarantors and lecturers
Literature
  • Recommended: Harlow E., Lane D. Antibodies a laboratory manual. Cold Spring harbor Lab. USA, 1988.
  • Recommended: Voet D., Voetová J. G. Biochemie. Victoria Publishing, Praha, 1990.
  • Recommended: auct. Fundamental Techniques in Cell Culture: A Laboratory Handbook. Sigma-Aldrich, 2007.
  • Recommended: Ferenčík M. Imunochémia. Alfa, Bratislava, 1989.
  • Recommended: McCullough K., Spier R. E. Monoclonal antibodies in biotechnology: theoretical and practical aspects. Cambridge University Press, Cambridge, UK, 1990.
  • On-line library catalogues
Time requirements

Activities

Time requirements for activity [h]

Preparation for the Exam

10
Total

10

Prerequisites – other information about course preconditions
Students should have a basic knowledge of immunology.
Competences acquired
Student should be able to (after attending the course): – characterise the immune response; – recall the principles of immunochemicals methods; – explain the differences between monoclonal, polyclonal and recombinant antibodies; – describe and interpret hybridoma technologies; – judge the advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies;
Teaching methods
  • Lecture
Assessment methods
  • Written exam