Principles and Applications of Molecular Biotechnology (LRR / MBT)

Course objectives:
To introduce latest industrial, biomedical, pharmaceutical and agricultural applications of microbial and cell cultures.
Requirements on student
Consistent with lecture content. Student should be able to discuss individual topics.
Scope, emergence and development of molecular biotechnology. Microbial diversity and microorganisms with uses in biotechnology. Recombinant DNA technology, principles of gene expression in prokaryotic and eukaryotic cells. Large-scale production of proteins from recombinant microorganisms. Production of protein and nucleic acids therapeutics, recombinant vaccines. Synthesis of enzymes, small biological molecules, antibiotics and biopolymers by recombinant microorganisms. Microbial insecticides (Bacillus thuringiensis toxin, baculoviruses). Bioremediation and biomass utilization.
Guarantors and lecturers
  • Basic: Glick B.R., Pasternak J.J., Patten Ch. Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition. ASM Press, USA, 2010. ISBN 978-1-55581-498-4..
  • On-line library catalogues
Time requirements


Time requirements for activity [h]


Preparation for the Exam




Prerequisites – other information about course preconditions
To know fundamentals of biochemistry and molecular biology.
Competences acquired
Student should be able to describe basic principles of molecular biotechnology, to describe principles of production of commercial products and therapeutics using genetically engineered microorganisms and cell cultures, and to describe the use of genetically engineered microorganisms for insect pest control,remediation and biomass utilization.
Teaching methods
  • Lecture
Assessment methods
  • Mark
  • Oral exam